After comparing TICRR expression in three HCC cells (Figure 10E), HepG2 and Hep3B cells were chosen for transfection with three independent siRNAs targeting TICRR. Based on knockdown efficiency, two siRNAs (si-TICRR-1 and si-TICRR-3) were employed in depletion of TICRR (Figure 10F). This evidence concerns the gene TICRR and hepatocellular carcinoma.