IDH1 and colorectal carcinoma: However, we examined the effects of Ag120 treatment for 72 h on intracellular 2-HG levels and found that the production of 2-HG in CRC cells is about 100–300 ng/ml (Figure 4A), which means CRC cells do not exist IDH1 mutation (Pollyea et al., 2014; Marina et al., 2021).