Following 2 h exposure to HTL-001, CD133 + cells (IC50 11.21 μM) were more sensitive to HTL-001 than CD133- cells (IC50 46.36 μM) and KNS42 parental cells (IC50 29.16 μM), Fig. 3C. We compared the sensitivity of HTL-001 in neurosphere-derived GBM CSCs grown in stem cell permissive conditions to that of their parental cell lines. The gene discussed is PROM1; the disease is glioblastoma.