NEFL and early-onset autosomal dominant Alzheimer disease: NfL324 [neurofilament light chain tryptic peptide GMNEALEK (NfL amino acids 324–331)] was 1.5-fold increased in Alzheimer’s disease compared with control (P = 0.001, Fig. 4F) and NfL530 was 1.7-fold increased (P = 0.0001, Fig. 4G).