The dual-luciferase reporter assay implied that the luciferase activity of WT-RNF38 3′UTR was obviously decreased in HCC cells with miR-377-3p mimic transfection, while the luciferase activity of MUT-RNF38 3′UTR was not affected by miR-377-3p (Figure 5b). The gene discussed is RNF38; the disease is hepatocellular carcinoma.