We found that hyperglycemia-mediated GLUT4 suppression occurred in the GLUT4 deletion constructs of pGLUT4–2000, −1600, −1400, −1200, −800, −600 and −400, while reporter suppression was partly restored in pGLU4–200 and −100 and completely restored in the pGLUT4–0 construct, suggesting that hyperglycemia-mediated transcriptional suppression is located in the range of −300 ~ −0 on the GLUT4 promoter (Figure 2g). Here, SLC2A4 is linked to Hyperglycemia.