Furthermore, m6A reader YTHDF2 was shown to promote the liver cancer stem cell phenotype and cancer metastasis by regulating OCT4 expression via m6A RNA methylation.48 However, attributed to the ambiguous cancer stem cell marker and the difficulties in the isolation and culture of cancer stem cell, most studies only investigated the effect of RNA methylation on cancer stem cell phenotype. This evidence concerns the gene POU5F1 and liver cancer.