The pyrosequencing results also determined that cg23487201 methylation level was significantly upregulated in ccRCC compared with AN tissue, and cg23487201 methylation level was significantly negatively correlated with APCDD1L-AS1 expression level (r=-0.7562, P=0.0011) (Fig. 5B). This evidence concerns the gene APCDD1L and nonpapillary renal cell carcinoma.