The second and third generation of TEM8-specific CAR-T cells targeting TEM8 on TNBC cell lines including MDA-MB-231, MDA-MB-436, MDA-MB-468 and HS578T and human breast tumor endothelial cell line HC6020 demonstrated cytotoxic effects in vitro and inhibition of tumor initiation in MDA-MB-468 xenografts143. The gene discussed is ANTXR1; the disease is neoplasm.