After knockdown of p53 with siRNA, the level of P-AKT reduced markedly in various human cancer cells, including colon cancer cells HCT116, SW480 (p53R273H) and pancreatic cancer cells PANC-1 (p53R175H) (Figure 2B-2D, Supplementary Figure 2B-2D). Here, TP53 is linked to familial pancreatic carcinoma.