To study AKT activation status in p53 wild-type and mutant cells, the phosphorylation level of AKT was detected, and we found P-AKT level increased significantly in p53 mutant cells (SW480, PANC-1, SKBR3), which promoted the occurrence and proliferation of cancer cells (Figure 2A, Supplementary Figure 2A). Here, TP53 is linked to cancer.