Taken together, our results provide novel mechanistic insight by which CLDN10 overexpression up-regulates the acetylation and expression levels of ATP5O protein, which leads to up-regulation of NDUFS2 protein, therefore suppressing the growth and metastasis of ccRCC by causing mitochondrial dysfunction (Fig. 8O). This evidence concerns the gene NDUFS2 and nonpapillary renal cell carcinoma.