Compared to Sham, ischemic stroke triggered a significant reduction of SPAK protein expression in the ipsilateral LVCP (ROI, Fig. 1b and c, p < 0.001) but did not significantly alter the expression of SPAK or NKCC1 proteins in the non-stroke contralateral (CL) LVCP (Fig. 1b and c, p > 0.05). Here, SLC12A2 is linked to ischemic stroke.