In a melanoma model, Liu et al. [105] reported that a ssRNA-Pim-3-small hairpin RNA (shRNA) dual-function vector could activate TLR7 by ssRNA fragments to stimulate antitumor immune response, such as activation of CD8+ T cells and NK cells and reduction of intratumoral Treg and MDSCs. This evidence concerns the gene TLR7 and melanoma.