Although the bioinformatics analysis showed that normal breast tissue presented the highest increase in Akt activation mediated by obesity, MCF-10A cells were discarded, since they did not show adequate activation of the IR-β/Akt/p70S6K pathway by stimulation with human serum (S3A Fig), nor could they differentiate between sera from different BMI (S4A Fig). Here, RPS6KB1 is linked to obesity due to melanocortin 4 receptor deficiency.