This study aimed to probe the possible biological functions and underlying mechanisms of circ_0004277 in AML cells, and with bioinformatics analysis, we hypothesized that circ_0004277 might participate in AML progression via inhibiting miR-134-5p and up-regulating single stranded DNA binding protein 2 (SSBP2). Here, SSBP2 is linked to acute myeloid leukemia.