Moreover, to gain further insights into the effects of glioblastoma HA synthesis on macrophages polarization, qRT-PCR and ELISA were respectively performed to measure the mRNA expression levels of M2 macrophages marker (CD163, CCL2, Arg1, IL1RA, TGFβ1) and the secretion of IL-10 by induced macrophages. Here, CD163 is linked to glioblastoma.