To gain insight into the mechanism by which NEDD9 depletion reduced glycolysis, we siRNA depleted NEDD9 expression in the A549 and H460 NSCLC cell lines for 48 h, then probed the expression and activity of the NEDD9 effectors FAK and SRC, and of proteins involved in glycolysis (Fig. 2A). Here, PTK2 is linked to non-small cell lung carcinoma.