Representative images of IHC staining are shown in Fig. 1A. Positive B7-H4 expression was detected in 132 of the CRC tissue specimens (132/225, 58.7%), and B7-H4 was expressed in the membrane, cytoplasm and nucleus of colorectal tumor cells (Fig. 1A). The gene discussed is VTCN1; the disease is colorectal neoplasm.