In two patients with no detectable CAR-T cells, one (patient #16) presented grade 1 CRS, significantly increased cytokines (Table S3) and obtained CR, furthermore, he had no CD19-positive B-cells in BM after CAR-T, we assumed that this case actually had expanded CAR-T cells whereas these cells could not be detected by FCM (no PCR data) due to an uncertain reason. This evidence concerns the gene CD19 and congenital rubella syndrome.