We therefore investigated the expression of the seven hepatokines uncovered by our proteomic analysis in the liver of a genetic mouse models of obesity, steatosis and IR/T2D, i.e., ob/ob mice (mice deficient for leptin) (Figure 4A and Figure S3) and db/db mice (mice deficient for leptin receptor) (Figure 4B and Figure S3). Here, LEP is linked to obesity due to melanocortin 4 receptor deficiency.