Analysis confirmed above results, as promoter methylation level of ENPP2 was increased in primary tumor tissues of BrCa patients in relation to normal (p < 1 × 10−12) as depicted in Figure 3A. Next, expression analysis showed downregulation of ENPP2 mRNA expression in primary tumor tissues in relation to normal tissues (p = 1.6 × 10−12) as depicted in Figure 3B. Similarly, protein expression analysis showed downregulation in primary tumors in relation to normal tissues (Figure 3E, p = 4.5 × 10−12). This evidence concerns the gene ENPP2 and neoplasm.