PKM and malignant colon neoplasm: Here, we explored the mechanism of kaempferol in reversing the drug resistance of colon cancer, and proved for the first time that kaempferol can up-regulate the expression of microRNA-326 (miR-326); we also showed that miR-326 inhibits the expression of PKM2 by directly targeting the 3′-UTR (untranslated region) of PKM2 or indirectly blocking the alternative splicing factor of PKM mRNA, and re-sensitizes 5-Fu drug-resistant colon cancer cells to 5-Fu by antagonizing glycolysis.