Moreover, the enhanced ER stress evidenced by the increasing expression of liver X-box binding protein 1 (XBP1), IRE1a, and the C/EBP homologous protein (CHOP) in HFD-fed BI-1−/− mice with NASH could also activate the NLRP3 inflammasome. This evidence concerns the gene DDIT3 and metabolic dysfunction-associated steatohepatitis.