Meanwhile, TUDCA could abolish the above changes caused by LPS, indicating that TUDCA suppressed NLRP3 inflammasome-induced pyroptotic death by inhibiting LPS-induced ER stress to improve the NASH model, and ER stress promoted NLRP3 inflammasome-induced pyroptosis. The gene discussed is NLRP3; the disease is metabolic dysfunction-associated steatohepatitis.