This was followed by a detailed analysis of gliomas, which entailed using SPME to assess the intra- and inter-tumor distribution of lipids in neoplastic lesions The lipidomic profile of these lesions was also assessed with respect to malignancy grade, isocitrate dehydrogenase mutation status, and the presence of 1p/19q codeletion. This evidence concerns the gene IDH3A and central nervous system cancer.