By flow cytometry, we found that CD83 expression significantly increased in SLN LCs after 24 h-stimulation with inflammatory cytokines (Figure 4f), as compared to both fresh SLN LCs (Figure 4d) and 24 h-culture without inflammatory cytokines (data not shown) (59.15%, range 47.11–77.25%; 19.29%, range 13.26–23.17%; and 19.94%, range 15.47–24.42%, respectively; p < 0.05), suggesting that SLN LCs are not irreversibly impaired by melanoma cells and immunosuppressive soluble factors. This evidence concerns the gene CD83 and melanoma.