An in vivo study on HCC rats treated with the crude cobra Naja haje oxiana venom causes an increase in ROS (reactive oxygen species) rate via the disruption of the liver mitochondria of mice, this disturbance can be related to either alteration, mitochondrial swelling and release of cytochrome c, which initiates apoptosis by activation of caspase 3 in HCC rats [41]. This evidence concerns the gene CASP3 and hepatocellular carcinoma.