We used the method to quantify the HPX glycoforms in serum samples of HCV-induced liver disease, and we demonstrate that the mLC-MS/MS-PRM assay offers substantially higher throughput compared to our reported workflow [11], maintains higher sensitivity of detection, and offers a high-throughput serologic assay (100 injections/day) for an improved screening of these glycopeptide biomarker candidates. The gene discussed is HPX; the disease is liver disorder.