Apart from the ELISA assay, there are other methods for the measurement of MMP levels that could have been constituted; for example, Western blot, dento-analyzer, time-resolved fluorescent immunoassay, etc. The relationship of hyperglycemia with MMP-9 levels can be assessed if random glucose levels are recorded for each subject together with their saliva samples collection. The gene discussed is MMP9; the disease is Hyperglycemia.