However, the performance of the examination depends on Prostate Specific Antigen (PSA) levels and the Gleason grade of the initial neoplasia, as per the study of Venkatesan et al. which reported 87.2% negative examinations in cases of PSA less than 0.5 ng/mL in lower grade tumor and 88.9% positive examinations in cases of PSA higher than 1.5 ng/mL in higher grade tumor [78]. Here, KLK3 is linked to neoplasm.