Because the AMPK-SREBP1 pathway plays an essential role in lipid metabolism, we explored whether the alleviating effect of TH-CS on NAFLD was associated with the expression of AMPK, the phosphorylation of AMPK, SREBP-1c and its downstream target genes FAS in the HFD-fed mice. This evidence concerns the gene FAS and metabolic dysfunction-associated steatotic liver disease.