We confirmed, by RT-qPCR, that pseudo-SARS-CoV-2 induced type I (IFNβ) and III (IFNλ1) IFNs expression, the first protection against viral infections, as well as the expression of interferon-stimulated genes (IFIT1, TRIM22, and MX2), which counteract viral replication, transcription, and translation in infected and uninfected cells and stimulate the adaptive immune response. This evidence concerns the gene IFNB1 and viral infectious disease.