iAstrocytes differentiated from iNPCs that were directly converted from mSOD1-ALS and sALS patient fibroblasts were then assessed for astrocyte-associated GFAP, Cx43 and Ki-67, as well as for inflammatory-associated miR-181b, miR-21, miR-155 and miR-146a. The gene discussed is GFAP; the disease is amyotrophic lateral sclerosis.