The t-NGS study demonstrated the existence of the β-Spectrin (SPTB) gene mutation (c.647G>A; p.Arg216Gln) in seven out of 11 family members and of a PKLR gene mutation (c.1706>a; p.Arg569Gln) in three of the seven members with the SPTB mutation, confirming the co-inheritance of HS and PKD in ID5, ID8, and ID9 (Figure 1). This evidence concerns the gene SPTB and histiocytic sarcoma.