IL6R and breast cancer: To evaluate whether miR-23c was able to negatively regulate the expression of endogenous IL-6R in breast cancer cell lines, we analyzed the levels of expression of IL-6R transcript by Real-time PCR and protein by cytofluorimetry and Western blotting in MDA-MB-468 and MDA-MB-231 cells transfected with the miR-23c mimic (Figure 7A–C).