MLANA and neoplasm: When tumor irrelevant vaccination against the SIV gag was combined with αCTLA-4 i.d., we found a significant increase in TILs from flow cytometry compared to the control and treated animals (no treatment, IgGb2 i.d., and IgGb2, i.d. + Melan-A VLP) (Figure 5C) without any change in PBMC lymphocytes (Supplementary Figure S3C).