To confirm that RNF5 is a candidate target for the treatment of NB and melanoma, we evaluated its expression in a panel of human NB (SH-SY5Y, SK-N-SH, IMR-32, HTLA-230) and human melanoma (MZ2-MEL, A2058, G-361, SK-MEL-28) cell lines by western blot. This evidence concerns the gene RNF5 and melanoma.