To validate the findings of the microarray analysis, the six genes identified (PPARD, BCL3, CD44, FOSB, MYC, and STAT1), along with POSTN and LUM, were assessed using RT-qPCR in 11 archival tissue biopsies from four severe asthmatics (AS), three asthmatics who developed lung cancer (AC), and four lung cancer (LC) patients. The gene discussed is LUM; the disease is laryngotracheoesophageal cleft.