IFNA1 and infection: To further characterize the role of the amino acid at G333 with respect to astrocyte infection and IFN induction, we isolated astrocytes from the brains of immunocompetent S129 mice at 5 days after intracranial inoculation of rHEP or rHEP333R. To estimate the incidence of rRABV infection in astrocytes in the whole brain, we quantified viral RNA levels in astrocytes sorted from the rRABV-infected brain, and the data were described relative to viral replication in the whole brain.