Neither DOX nor ANGII alone was sufficient to significantly reduce the cardiac function in mice (Figures 3A–D); however, juvenile exposure to DOX followed by adult-onset ANGII-induced hypertension caused a significant deterioration in cardiac function parameters as shown by a decrease in cardiac output (Figure 3B), stroke volume (Figure 3C), and ejection fraction (Figure 3D). Here, AGT is linked to Hypertension.