NEK6 and renal cell carcinoma: To further establish our hypothesis that FAM13A-AS1 functions by negatively regulating miR-326, we used shRNA to knockdown FAM13A-AS1 in RCC cells, which resulted in a significant reduction in NEK6 RNA and protein levels, resembling the effect of transfection with miR-141-3p mimic (Figures 7A,B).