FLT1 and melanoma: Graziani et al. developed an anti-VEGFR-1 mAb (D16F7) by immunizing BALB/C mice which markedly inhibited angiogenesis, endothelial cell migration and intracellular signal transduction in melanoma and glioblastoma (Graziani et al., 2016; Atzori et al., 2017).