To explore the effects of EGFR-TKIs on autophagy in NSCLC cells, the expression of p62 and LC3 were observed by western blotting (Figure 1B) or based on GFP+RFP+ and GFP−RFP+ puncta in cells using confocal microscopy (Figure 1C). The gene discussed is MAP1LC3A; the disease is non-small cell lung carcinoma.