IFNG and neoplasm: We further purified the T cells from spleen and treated them with either Ad.5-null or Ad.5-mda-7. Treatment with Ad.5-mda-7 up regulated IFN-γ production in purified T cells suggesting that the regulation of IFN-γ is mediated by the tumor microenvironment and not B16 tumor cells (Supplementary Figure 5B).