In which, PPAR signaling pathway, IL-17 signaling pathway were highly enriched in SR vs SC and SS vs SC after C. perfringens infection, antigen processing and presentation, intestinal immune network for IgA production, hematopoietic cell lineage was specifically significantly in SS vs SC, RIG-I-like receptor signaling pathway were specifically significantly enriched in SS vs SR, these identified significantly signaling pathways were involved in the process of piglet spleen immune responses against C. perfringens infection. This evidence concerns the gene PPARA and infection due to clostridium perfringens.