Our immunostaining results showed that the number and ratio of iNOS+Iba-1+ microglia increased in the SDH of T1DM mice compared with control mice (Figure 3(a); P = 0.002 for the number of iNOS+Iba-1+ cells, P = 0.0024 for iNOS+ ratio in Iba-1+ microglia, n = 4). The gene discussed is NOS2; the disease is type 1 diabetes mellitus.