Noticeably, treatment with rIGF-1 increased the IGF-1+ ratio in Iba-1+ cells (Figures 5(c) and 5(e), P = 0.0018, n = 5) and reduced iNOS+Iba-1+ microglia in the SDH of T1DM mice on D14 (Figures 5(f) and 5(g), P = 0.0003, n = 5). This evidence concerns the gene NOS2 and type 1 diabetes mellitus.