However, indeed, significant number of NAFLD-related genes (such as IGF1, IGFBP2 and PPARG in genomic DNA and MT-ND6 on mitochondrial DNA) underwent hypermethylation with the progression of NAFLD in rodent models as wells as in NAFLD patients 17, 18. Here, IGF1 is linked to metabolic dysfunction-associated steatotic liver disease.