MPEG1 and infection: To investigate whether the different infection conditions alter the kinetic of macrophage subsets recruitment and activation, we took advantage of the Tg(mpeg1:mCherry-F/tnfa:eGFP-F) zebrafish line, followed by confocal microscopy analysis at 1, 3, 6, 24, 48 and 72 hpA (Figure 4A).