To determine whether (+)-JQ1 may mitigate colitis onset in vivo, we treated adult Il10-/- mice previously housed under germ free (GF) conditions with (+)-JQ1 (I.P. injections) followed by colonization with slurries made using fecal matter from WT C57BL/6J mice raised in specific pathogen free conditions (Figure 4A). This evidence concerns the gene IL10 and colitis.