Perhaps even more significantly, we further demonstrated that phosphorylation and total abundance of S6 ribosomal and Akt proteins were not elevated, and autophagy inhibition is maintained in THP-1-derived macrophages infected with Mu::Tn118C′ (+aTCN), as observed in Mu1615 and Mu::Tn118 infection (Figures 5E, F, and Supplementary Figure 6). This evidence concerns the gene AKT1 and infection.