To evaluate the effect of IFITM3 on PEDV and TGEV infection, porcine IPEC-J2 cells were transfected with si-ssc-IFITM3s or pLV-sIFITM3-Flag for 48 h, followed by infection with PEDV or TEGV (MOI = 1). This evidence concerns the gene IFITM3 and infection.